Positively charged nylon membrane
A positively charged nylon membrane with a uniform distribution of positive charge density ensures the highest sensitivity and lowest background signal. It has an extremely long durability and can withstand multiple repeat hybridization tests. Very satisfactory results in both chemiluminescence and radioactivity detection systems, suitable for Southern/Northern/Dot/Slot Blot/Colony Lift experiments.
Nitrocellulose membrane / PVDF membrane
There are a variety of solid phase carriers for Western blotting, and nitrocellulose membranes and PVDF (polyethylene oxide) membranes are most commonly used. The nitrocellulose membrane has the advantages of strong binding ability, no activation of the membrane, shallow background, multiple immunoassays and long life of functional groups, but it is extremely easy to break and difficult to handle. PVDF membranes are most commonly used in the preparation of peptides for chemical analysis of proteins. It has superior protein binding capacity and high signal-to-noise ratio, so there is no loss of protein due to repeated use of the probe. In addition, the open pore structure of the membrane makes it easier to bind to the adsorbed protein. It can be used for Western hybridization, dot/slot hybridization, protein sequencing, and MS analysis.
Suitable for Southern/Northern/Dot/Slot Blot/Colony Lift experiments and Western, protein sequencing and MS analysis.